Human gamma-thrombin

Human Gamma-thrombin (Ref. HCGT-0021) is a purified enzyme derived from human α-thrombin by limited digestion. Formulation: 100 mM Na3PO4, 0.1% PEG, pH 6.5, Purity > 95% by SDS-PAGE.

Human Gamma-thrombin (Ref. HCGT-0021) is a purified enzyme derived from human α-thrombin by limited digestion. This enzyme exhibits a residual coagulant activity of less than 1% compared to active α-thrombin, making it ideal for research applications requiring low enzymatic activity, such as studying coagulation mechanisms, cell signaling, and protein-protein interactions.

Technical Specifications:

• Molecular Weight: 35,400 Da
• Specific Activity: <1% of α-thrombin activity
• Concentration: Varies with each lot
• Buffer: 100 mM Sodium Phosphate, 0.1% PEG, pH 6.5
• Purity: >95% by SDS-PAGE
• Extinction Coefficient: E1% = 18.3 at 280 nm, 1 cm
• Recommended Storage: -80/-40°C
• Shipped Volume: 0.1 mg or 1 mg, depending on the reference

Recommended Applications:

• Coagulation Studies: Research on blood coagulation mechanisms without triggering the full coagulation cascade.
• Cell Signaling: Analysis of thrombin-dependent signaling pathways in various cell types.
• Molecular Screening: Identification and validation of thrombin-specific inhibitors.
• Protein-Protein Interactions: Study of interactions between thrombin and its receptors or other target proteins.

• The vast majority of enzymes are pure (no additives): purity > 95% by SDS-PAGE.
• Expiration date: one year from delivery.
• Bulk delivery available.
• All references benefit from volume-based pricing.

An enzyme is a protein that catalyzes a biochemical reaction. It converts a substrate into a product. Each enzyme has a structure tailored to its function, and its activity depends on an optimal temperature and pH. Gamma-thrombin is obtained by cleavage of the B2 chain of beta-thrombin at the Lys190-Gly191 position, resulting in the B4 and B5 fragments.