Pefachrome® FXa 5277

• Application: Chromogenic peptide substrate for Factor Xa.
• Formula: CH₃SO₂-D-Leu-Gly-Arg-pNA · AcOH
• Packaging: 25 mg
• Status: RUO
• Storage: 2°C – 8°C
• Km: 233 µM (human thrombin) / 154 µM (bovine thrombin)
• Vmax: 0.736 µM/min (human) / 0.141 µM/min (bovine)
• kcat: 5643 min⁻¹ (human) / 1080 min⁻¹ (bovine)

Informations ;

The line of chromogenic peptide substrates is a range of high quality substrates, which allow to test protease serines.
They target enzymes involved in coagulation and fibrinolysis such as thrombin, Factor Xa, Factor XIIa, kallicrein, activated C protein, plasmin and plasminogen-SK. These are synthetic peptides that react with proteolytic enzymes by releasing a colour that can be followed by spectrophotometry and whose intensity is proportional to the proteolytic activity of the enzyme.Typically, such chromogenic substrates are composed of 3 to 5 natural or artificial amino acids. Their structures can be protected in N-terminal to reduce undesirable degradation by aminopeptidases.
Their C-terminal ends are modified so that, during the cleavage of the amide bond, a chromogenic group is released. The most commonly used group is p-nitroaniline (pNA), which absorbs light at a wavelength of 405 nm.

Package Inserts, certificate of analysis supplied.
Material safety Data Sheet (MSDS) supplied.
Prolonged stability following reconstitution (> 3 months).