Factor IXa Calibrator
| 5-9599 | Vial | 10 x 2.0 mL |
Purified preparation of Factor IXa for the ROX FIX-A kit, calibrated against the international standard WHO.
Description of the Factor IXa Calibrator
Calibration plasma for the determination of FIXa in colorimetry, it can be used directly without dilution after reconstitution.
Method / Application
The ROX FIX-A is a chromogenic enzymatic assay kit for the determination of very low amounts of FIXa in human FX concentrates.
The results are expressed in IU. The sensitivity of the assay is 0.1 mIU / mL. This method is based on the activation of FX by FIXa (considered a contaminant in FIX concentrate) in the presence of FVIII, thrombin, phospholipids and calcium ions.
The amount of FXa generated is determined by hydrolysis of a chromogenic substrate for FXa. The quantity of para-nitroaniline released during this hydrolysis and measured at 405 nm is proportional to the concentration of FIXa in the reaction medium.
Informations
FIX is a vitamin K dependent glycoprotein synthesized by the liver. FIX can be activated to FIX in FIXa by FXIa or by FVIIa in the presence of phospholipids and calcium.
A person who is deficient in FIX has hemophilia B.
