Fluorogenic substrate ANSN for Factor XIa (LPR)

MW(Da) : 721.74
Km FIIa : 0.5 µM - Kcat : 19 s-1
Km FVIIa : 300 µM - Kcat : 0.07 s-1
Km FVIIa/FT : 300 µM - Kcat : 4.5 s-1
Km FXa : 171 µM - Kcat : 3.3 s-1
Km FXIa : 75 µM - Kcat : 53 s-1
Km PCa : 45 µM - Kcat : 52 s-1
Km t-PA : 98 µM - Kcat : 0.31 s-1

The kinetic properties identified on the following page will aid in the selection of an appropriate substrate.
The ANSN-based substrates are provided as 10 mM stock solutions in DMSO.
Assays are typically conducted in physiologic buffers containing Hepes or Tris, with substrate concentrations ranging from 1 to 100 µM.
The relative change in fluorescence is monitored at a 470 nm emission wavelength with a 352 nm excitation wavelength.
Light artifacts can be minimized by employing a 390 to 450 nm long-pass cutoff filter in the emission beam.
The stock substrate solutions in DMSO could remain frozen at 4° C or colder, and should be protected from light.
Under these conditions the compounds will remain stable for over one year.