Fluorogenic substrate ANSN for Factor XIa (EGR)

MW(Da) : 724.6
Km FIIa : 100 µM - Kcat : 2.5 s-1
Km FXa : 110 µM - Kcat : 0.2 s-1
Km FXIa : 225 µM - Kcat : 82 s-1
Km PCa : 440 µM - Kcat : 17 s-1

The kinetic properties identified on the following page will aid in the selection of an appropriate substrate. The ANSN-based substrates are provided as 10 mM stock solutions in DMSO.
Assays are typically conducted in physiologic buffers containing Hepes or Tris, with substrate concentrations ranging from 1 to 100 µM.
The relative change in fluorescence is monitored at a 470 nm emission wavelength with a 352 nm excitation wavelength. Light artifacts can be minimized by employing a 390 to 450 nm long-pass cutoff filter in the emission beam.
The stock substrate solutions in DMSO could remain frozen at 4° C or colder, and should be protected from light. Under these conditions the compounds will remain stable for over one year.