Fluorogenic substrate ANSN for plasmin

Molecular weight (Da) : 786.6
Concentration : 7.9 mg/mL
Km : 130 µM
Kcat : 3.7 s-1
Buffer formulation : Dimethyl sulfoxide (DMSO)

The kinetic properties identified on the following page will aid in the selection of an appropriate substrate. The ANSN-based substrates are provided as 10 mM stock solutions in DMSO. Assays are typically conducted in physiologic buffers containing Hepes or Tris, with substrate concentrations ranging from 1 to 100 µM. The relative change in fluorescence is monitored at a 470 nm emission wavelength with a 352 nm excitation wavelength. Light artifacts can be minimized by employing a 390 to 450 nm long-pass cutoff filter in the emission beam. The stock substrate solutions in DMSO could remain frozen at 4° C or colder, and should be protected from light. Under these conditions the compounds will remain stable for over one year.