Fluorogenic substrate ANSN for thrombin
| 9-SN-20 | Vial | 1 mg |
Sequence : Boc-L-FPR-ANSNH-C₂H₅
Formulation : Dimethyl sulfoxide (DMSO)
Description of the Fluorogenic substrate ANSN for thrombin
MW(Da) : 750.9
Km FIIa : 17 µM - Kcat : 58 s-1
Km FXa : 100 µM - Kcat : 0.31 s-1
Km PCa : 40 µM - Kcat : 2.2 s-1
Km t-PA : 47 µM - Kcat : 0.011 s-1
Informations
The kinetic properties identified on the following page will aid in the selection of an appropriate substrate.
The ANSN-based substrates are provided as 10 mM stock solutions in DMSO. Assays are typically conducted in physiologic buffers containing Hepes or Tris, with substrate concentrations ranging from 1 to 100 µM.
The relative change in fluorescence is monitored at a 470 nm emission wavelength with a 352 nm excitation wavelength.
Light artifacts can be minimized by employing a 390 to 450 nm long-pass cutoff filter in the emission beam. The stock substrate solutions in DMSO could remain frozen at 4° C or colder, and should be protected from light. Under these conditions the compounds will remain stable for over one year.
