Vitronectin (Vn) is an adhesive glycoprotein, synthesized by the liver, released in plasma and present in the extracellular matrix. Vn binds PAI-1.
This complex fully activates PAI-1, unlike PAI-1 in solution, where it does not appear to be stable and inactive.
Vn therefore seems to regulate the enzymatic specificity of PAI-1, by stabilizing it. Decreased Vn levels occur in DICs and liver disease (cirrhosis). Vn deposition is associated with atherosclerotic lesions.
The test is based on the quantification of vitronectin using 2 antibodies; the first monoclonal to bind Vn and the second polyclonal coupled to POX for detection. (Specialized hemostasis)
- Stability 3 months after opening.
- Reaction time 240 minutes.
- Dosage sensitivity ranging from 0 to 400% vitronectin.
- 12 breakable ELISA strips (12 x 8 wells)
- 2 adhesives for ELISA plate
- 1 vial x conjugated antibody-POX
- 1 vial x TMB chromogenic substrate (12 mL)
- 1 bottle x stop solution (15 mL)
- 1 vial x 2.5x concentrated dilution buffer (20 mL)
- 1 vial x POX dilution buffer (12 mL)
- 1 vial x 12.5x wash buffer concentrate (20 mL)
- 1 vial x lyophilized calibrator plasma
