OLIGOBIND® Thrombin Activity Assay

The conversion of prothrombin to thrombin is a key event in thrombus formation. Thrombin is a serine protease that acts on a wide variety of substrates during the clotting process.
Thrombin generated in vivo can be assessed indirectly by measuring the fragment of prothrombin F1.2, an activating peptide generated during the conversion of prothrombin to thrombin, or thrombin-antithrombin complexes (TAT), formed during inactivation of thrombin by its major inhibitor present in plasma.
However, due to differential accumulation in the circulation, these parameters do not reflect the current state of functional active thrombin in vivo.