Mouse monoclonal antibody against human uPAR
| 26-ADG3937 | Flacon | 1 x 250 µg |
The monoclonal antibody ADG3937 (clone HD-UPAR-13.1.1) is a murine IgG1 monoclonal antibody that recognizes domains 2+3 of the human urokinase receptor (uPAR). It binds with high affinity to both uPAR and uPA/uPAR complexes.
Description of the Mouse monoclonal antibody against human uPAR
Properties
ADG3937 is non-inhibitory; it does not prevent uPA from binding to uPAR. Pre-incubation with various uPAR preparations (CHO cell line, U937 cells) can completely inhibit ADG3937 binding to uPAR. Cross-reactivity with uPAR from other species has not been determined.
Presentation
Screw-cap vial containing 250 µg of purified antibody in PBS pH 7.4 with 0.01% ProClin300. IgG concentration: 1 mg/mL.
Storage and Stability
Store the antibody at 2–8°C. For long-term storage, aliquot and keep at –20°C or below. Avoid repeated freeze-thaw cycles.
Applications
A. Immunohistochemistry / Flow Cytometry
ADG3937 is suitable for staining formalin-fixed, paraffin-embedded tissue sections. It also stains freshly frozen, PMA-stimulated U937 cells fixed in formalin for flow cytometry. Recommended dilution: 1:100 (10 μg/mL).
B. uPAR Immunoprecipitation
Using a 5:1 molar ratio, ADG3937 precipitates various uPAR preparations, showing a broad band between 39–66 kDa on SDS-PAGE due to extensive N-linked glycosylation. Enzymatic deglycosylation reduces this to a single sharp band at 29 kDa.
C. Western Blot
ADG3937 can be used for Western blotting. Under non-reducing conditions, 2 μg/mL of ADG3937 can detect up to 50 ng of uPAR.
Informations
Belonging to the serine protease family, uPA activates plasminogen to convert it into plasmin, an enzyme responsible for fibrin degradation. It plays a key role in the clot dissolution phase during fibrinolysis.
