Fluorogenic substrate ANSN for PCa

MW(Da) : 746.98
Km FIIa : 19 µM - Kcat : 0.055 s-1
Km FVIIa : 42 µM - Kcat : 0.007 s-1
Km FVIIa/FT : 170 µM - Kcat : 1.6 s-1
Km FXa : 19 µM - Kcat : 0.055 s-1
Km PCa : 3.9 µM - Kcat : 2.1 s-1

The kinetic properties identified on the following page will aid in the selection of an appropriate substrate. The ANSN-based substrates are provided as 10 mM stock solutions in DMSO. Assays are typically conducted in physiologic buffers containing Hepes or Tris, with substrate concentrations ranging from 1 to 100 µM.
The relative change in fluorescence is monitored at a 470 nm emission wavelength with a 352 nm excitation wavelength.
Light artifacts can be minimized by employing a 390 to 450 nm long-pass cutoff filter in the emission beam. The stock substrate solutions in DMSO could remain frozen at 4° C or colder, and should be protected from light.
Under these conditions the compounds will remain stable for over one year.