HEMOSTASIS COAGULATION RESEARCH REAGENTS ENZYMES THROMBIN (FIIA)

Human gamma-thrombin

Structure: 4 chains (A, B1, B5 and B4) with a disulfide bridge between peptide A and peptide B5.
Formulation : 100 mM + 0,1% PEG

Human Gamma-thrombin (Ref. HCGT-0021) is a purified enzyme derived from human α-thrombin by limited digestion. Formulation: 100 mM Na3PO4, 0.1% PEG, pH 6.5, Purity > 95% by SDS-PAGE.

Human Gamma-thrombin (Ref. HCGT-0021) is a purified enzyme derived from human α-thrombin by limited digestion. This enzyme exhibits a residual coagulant activity of less than 1% compared to active α-thrombin, making it ideal for research applications requiring low enzymatic activity, such as studying coagulation mechanisms, cell signaling, and protein-protein interactions.

🧪 Technical Specifications:

Molecular Weight: 35,400 Da
Specific Activity: <1% of α-thrombin activity
Concentration: Varies with each lot
Buffer: 100 mM Sodium Phosphate, 0.1% PEG, pH 6.5
Purity: >95% by SDS-PAGE
Extinction Coefficient: E1% = 18.3 at 280 nm, 1 cm
Recommended Storage: -80/-40°C
Shipped Volume: 0.1 mg or 1 mg, depending on the reference

💡 Recommended Applications:

Coagulation Studies: Research on blood coagulation mechanisms without triggering the full coagulation cascade.
Cell Signaling: Analysis of thrombin-dependent signaling pathways in various cell types.
Molecular Screening: Identification and validation of thrombin-specific inhibitors.
Protein-Protein Interactions: Study of interactions between thrombin and its receptors or other target proteins.

Advantages

The vast majority of enzymes are pure (no additives): purity > 95% by SDS-PAGE.
Expiration date: one year from delivery.
Bulk delivery available.
All references benefit from volume-based pricing.

Informations

An enzyme is a protein that catalyzes a biochemical reaction. It converts a substrate into a product. Each enzyme has a structure tailored to its function, and its activity depends on an optimal temperature and pH. Gamma-thrombin is obtained by cleavage of the B2 chain of beta-thrombin at the Lys190-Gly191 position, resulting in the B4 and B5 fragments.

Documentation

Download the product sheet

Price list, safety data sheets and notices are accessible to our registered customers.

References

9-HCGT-0021	Vial	100 µg
9-HCGT-0021-1	Vial	1 mg

All enzymes are accompanied by certificates of analysis detailing the appropriate storage conditions. A brief centrifugation of the enzymes in their original packaging will help recover the sample completely at the bottom of the tube. Never leave protein solutions at room temperature for extended periods. High temperatures can accelerate protein degradation.

Avoid storing or maintaining diluted protein samples for prolonged periods. In general, purified proteins are more stable in concentrated form. Many proteins are inherently "adhesive." To prevent protein loss due to adsorption, extremely diluted protein samples should be prepared in buffers containing excipients such as bovine serum albumin (BSA), polyethylene glycol (PEG), Prionex, or gelatin. Prionex is a highly effective replacement for BSA.

	These kits are manufactured in accordance with the 98/79 EC directive for in vitro diagnostic devices. Only CE marked products can be used for diagnostic applications in Europe.
	These kits are intended for in vitro diagnostic use.
	These kits are for research use only and are not intended to be used for diagnostic procedures.
	Federal Drug Administration, FDA validates diagnostic kits for in vitro diagnostic use in the United States.
	Biological risk products.
	Storage between 2 and 8 ° C.
	Reactive in liquid form.
	Reactive in lyophilized form.
	Reactive in frozen form.
	Stability after opening at 2-8 ° C.
	Products that can be refrozen.
	Stability 12 months after refreezing at -20 ° C.
	Manufacturer.
	Importer
	Distributor.

HEMOSTASIS COAGULATION RESEARCH REAGENTS ENZYMES THROMBIN (FIIA)