» Fluorogenic substrate ANSN for thrombin

HEMOSTASIS COAGULATION RESEARCH ANSN FLUOROGENIC SUBSTRATES FLUOROGENIC ANSN SUBSTRATES FOR THROMBIN (FIIA)

Fluorogenic substrate ANSN for thrombin

Sequence : Boc-L-FPR-ANSNH-C₂H₅
Formulation : Dimethyl sulfoxide (DMSO)

MW(Da) : 750.9
Km FIIa : 17 µM - Kcat : 58 s-1
Km FXa : 100 µM - Kcat : 0.31 s-1
Km PCa : 40 µM - Kcat : 2.2 s-1
Km t-PA : 47 µM - Kcat : 0.011 s-1

Reference	9-SN-20
Presentation	Vial
Format	1 mg
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Price list, safety data sheets and notices are accessible to our registered customers.

Reference	Presentation	Format	Quote	Product sheet
9-SN-20	Vial	1 mg

Price list, safety data sheets and notices are accessible to our registered customers.

The kinetic properties identified on the following page will aid in the selection of an appropriate substrate.
The ANSN-based substrates are provided as 10 mM stock solutions in DMSO. Assays are typically conducted in physiologic buffers containing Hepes or Tris, with substrate concentrations ranging from 1 to 100 µM.
The relative change in fluorescence is monitored at a 470 nm emission wavelength with a 352 nm excitation wavelength.
Light artifacts can be minimized by employing a 390 to 450 nm long-pass cutoff filter in the emission beam. The stock substrate solutions in DMSO could remain frozen at 4° C or colder, and should be protected from light. Under these conditions the compounds will remain stable for over one year.

Substrates containing the fluorescent reporter group 6-amino-1-naphthalene-sulfonamide (ANSN) are useful compounds for monitoring the enzyme activity of various serine proteases. In this class of is compounds, the ANSN reporter group linked (in the R1 position) to short tri-peptide sequences.
The peptide sequences are designed to optimize the interaction between the enzyme and substrate. Additional components which may be added to the R2 and R3 positions reflect changes in the P’ subsite positions, and generally affect the kinetic parameters of the substrates. Compounds which are effective substrates are hydrolyzed between the tri-peptide and the ANSN group. Once cleaved from the peptide moiety, the ANSN group exhibits about a 1000 fold increase in relative fluorescence.

Fluorogenic substrate ANSN for thrombin
Fluorogenic substrate ANSN for thrombin and FVIIa

Cryopep Catalogue
Thrombosis Haemostasis 2024

Cryopep Catalogue
Haemostasis Research 2024

Cryopep ANSN FLUOROGENIC SUBSTRATES

List of abbreviations

MW(Da) : 703.73
Km FIIa : 2 µM – Kcat : 110 s-1
Km FVIIa : 89 µM – Kcat : 0.019 s-1
Km FVIIa/FT : 52 µM – Kcat : 0.76 s-1
Km FXa : 160 µM – Kcat : 3.3 s-1
Km FXIa : 520 µM – Kcat : 92 s-1
Km PCa : 54 µM – Kcat : 72 s-1
Km t-PA : 110 µM – Kcat : 0.71 s-1

	These kits are manufactured in accordance with the 98/79 EC directive for in vitro diagnostic devices. Only CE marked products can be used for diagnostic applications in Europe.
	These kits are intended for in vitro diagnostic use.
	These kits are for research use only and are not intended to be used for diagnostic procedures.
	Federal Drug Administration, FDA validates diagnostic kits for in vitro diagnostic use in the United States.
	Biological risk products.
	Storage between 2 and 8 ° C.
	Reactive in liquid form.
	Reactive in lyophilized form.
	Reactive in frozen form.
	Stability after opening at 2-8 ° C.
	Products that can be refrozen.
	Stability 12 months after refreezing at -20 ° C.
	Manufacturer.
	Importer
	Distributor.

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HEMOSTASIS COAGULATION RESEARCH ANSN FLUOROGENIC SUBSTRATES FLUOROGENIC ANSN SUBSTRATES FOR THROMBIN (FIIA)