A complex web of biochemical and physical reactions between platelets and clotting factors at the site of vascular injury is required to achieve hemostasis.
Under flow conditions, platelet activation and coagulation processes are dynamically intertwined with each other affected by platelets, coagulation factors and their various inhibitors and activators.
Whole blood is perfused at a constant flow rate at 37°C through the flow chamber pre-coated with tissue thromboplastin and collagen. Changes of flow pressure are monitored by the pressure transducer located upstream in the chamber.
Thrombus formation within the flow chamber increases flow resistance causing the pressure to increase. OST (Occlusion Start Time) is the lag time for the flow pressure to reach 10 kPa due to partial occlusion of the capillary. OT (Occlusion Time) is the lag time for the flow pressure to reach 60 kPa from baseline pressure.
The AUC (Area Under the Curve) is the area under the flow pressure vs. time curve and is related to overall thrombus formation. Primary result is generated as AUC.
The HD chip has a 50 µm deep flow chamber coated with collagen and tissue thromboplastin, and mimics blood flow in vivo with a shear stress of 1200 / s, which represents shear stresses to the vessel wall arterial.
Ready to use.
