HEMOSTASIS COAGULATION RESEARCH REAGENTS COFACTORS THROMBOMODULIN
Rabbit lung thrombomodulin
Buffer formulation: 20 mM Tris; 150 mM NaCl, 0.05% PDOC (polidocanol), pH 7.4
Purified rabbit lung
V500 to 1 800 units/mg
Molecular weight (Da): 74 000
Extinction coef.: 8.8
Structure: single chain, hydrophobic domain in NH2-terminal, 6 EGF domains, 1 domain rich in O-glycosylation, 1 transmembrane domain and a cytoplasmic domain in COOH-terminal.
RABTM is not going to be very stable once it is thawed. It should be kept on ice and used within 2 hrs of thawing. After thawing it can be aliquoted and refrozen and retain its stability - Don't that multiple times though. We suggest making aliquots that are > 10 uL in volume or the protein will lyophilize.
Advantages
The vast majority of coFactors is pure (without additives) with > 95 % purity SDS-PAGE. Expiration date of one year from delivery Delivery in large quantities Discount according to quantities
Informations
Purified rabbit lung endothelial cell surface protein co-factor for protein C activation as the thrombin-thrombomodulin-Ca²+ complex. Rabbit lung thrombomodulin is a more efficient activator of human protein C than is human thrombomodulin. It is an essential reagent for valid assays of protein C functional activity in plasma.
The thrombomodulin is purified by a multi-step process that includes detergent solubilization from rabbit lung, salt fractionation, ionexchange chromatography and thrombin-sepharose affinity chromatography (Esmon NL, et al., Isolation of a membrane-bound cofactor for thrombin-catalyzed activation of protein C, Journal of Biological chemistry, 1982, 257:859-864).
In protein C activation, thrombomodulin must be used at a concentration such that all the thrombin remains complexed in order to avoid clotting
(Francis Jr. RB, A simplified PTT-based protein C activity assay using the thrombin-thrombomodulin complex, Thrombosis Research, 1986, 37:337-344).
Documentation
Download the product sheetPrice list, safety data sheets and notices are accessible to our registered customers.
References
| 9-RABTM-4202 | Vial | 50 µg |
| 9-RABTM-4202-1 | Vial | 1 mg |
