Fluorogenic substrate ANSN for t-PA

MW(Da) : 782.92
Km FIIa : 3.7 µM - Kcat : 44 s-1
Km FVIIa : 50 µM - Kcat : 0.008 s-1
Km FVIIa/FT : 217 µM - Kcat : 0.88 s-1
Km t-PA : 71 µM - Kcat : 1.03 s-1

The kinetic properties identified on the following page will aid in the selection of an appropriate substrate. The ANSN-based substrates are provided as 10 mM stock solutions in DMSO.
Assays are typically conducted in physiologic buffers containing Hepes or Tris, with substrate concentrations ranging from 1 to 100 µM.
The relative change in fluorescence is monitored at a 470 nm emission wavelength with a 352 nm excitation wavelength.
Light artifacts can be minimized by employing a 390 to 450 nm long-pass cutoff filter in the emission beam.
The stock substrate solutions in DMSO could remain frozen at 4° C or colder, and should be protected from light. Under these conditions the compounds will remain stable for over one year.