Belonging to the serine protease family, u-PA activates plasminogen to convert it into plasmin, an enzyme allowing the degradation of fibrin.
It intervenes in the phases of dissolution of the clot during fibrinolysis.
First, the functional u-PA assay is performed using Glu-plasminogen and a low molecular weight plasmin substrate. Secondly, the ELISA plate is washed and then a monoclonal antibody specific to u-PA, recognizing free u-PAs and complexed with inhibitors, is used. It is revealed by peroxidase. (Specialized hemostasis)
- Stability 3 months after opening.
- Reaction time 160 minutes then 140 minutes.
- Antigen : sensitivity of the assay ranging from 0 to 10 ng / mL u-PA.
- Activity : sensitivity of the assay ranging from 0 to 1 U / mL of u-PA.
- 12 x 8-well breakable ELISA strips coated with monoclonal anti-u-PA antibody
- 1 vial x biotinylated human u-PA polyclonal antibody
- 1 vial x TMB chromogenic substrate (12 mL)
- 1 bottle x stop solution (15 mL)
- 1 vial x dilution buffer (20 mL)
- 1 vial x POX dilution buffer (12 mL)
- 1 vial x wash buffer (80 mL)
- 1 vial x detection dilution buffer (20 mL)
- 1 vial x lyophilized u-PA calibrator
- 1 vial x streptavidin peroxidase (POX) solution
- 1 vial x plasminogen activator detection