Tissue plasminogen activator (t-PA) is a protein involved in breaking down the blood clot. It is a serine protease found in the endothelial cells that line the blood vessels.
Like any enzyme, it converts plasminogen into plasmin, the main blood clot lysis enzyme.
Due to its lysis activity, t-PA is used in clinical medicine to treat cerebral embolism and thrombosis.
Its use is contraindicated in cases of cerebral hemorrhage or head trauma.
The assay can be performed on citrate, EDTA or CTAD. The ELISA method uses 2 monoclonal antibodies.
The system detects both complexed and uncomplexed t-PA from a cutoff of 1 ng / mL.
- Stability 2 months after opening.
- Reaction time 140 minutes.
- The use of EDTA increases the DO signal.
- Sensitivity of the assay ranging from 0 to 30 ng / mL for t-PA.
- 12 breakable strips of 8 wells coated with an anti-t-PA monoclonal antibody
- 2 adhesives for ELISA plate
- 1 vial x anti-t-PA monoclonal antibody coupled to peroxidase (POX), 0.3mL
- 1 vial x TMB chromogenic substrate (12 mL)
- 1 bottle x stop solution (12 mL)
- 1 vial x wash buffer concentrate (80 mL)
- 1 vial x incubation buffer (90 mL)
- 1 vial x sample dilution buffer (20 mL)
- 5 vials x freeze-dried calibrators
- 1 vial x lyophilized high control plasma
- 1 vial x lyophilized low control plasma
