Factor VIII is a glycoprotein mainly synthesized by the liver. It circulates in the plasma as bound to vWF which protects it from rapid proteolytic degradation.
It is activated by FXa or thrombin in FVIIIa which will complex with FIXa in the presence of phospholipids to activate FX in FXa.
A patient who is deficient in FVIII has hemophilia A.
CRYOcheck™Chromogenic Factor VIII is used to identify FVIII deficiency and helps in the management of hemophilia A in people 2 years of age and older.
- Linearity: 0-200%
- Detection limit: 0.5% (activity%)
- Accuracy <4%
- 24 hours stability of reagents on analyzers
- Reagents refreezable 30 days at -70 ° C
- 4 vials of reagent 1 (bovine FX + fibrin inhibitor) (1.25 mL)
- 4 vials of reagent 2 (human FIIa, human FIXa, Ca ++ , phospholipids) (1.25 mL)
- 4 vials of reagent 3 (FXa substrate + thrombin inhibitor) (1.25 mL)
- 4 vials of Tris-BSA Dilution Buffer (7 mL)
