Tissue factor (FT) is a transmembrane glycoprotein which is primarily responsible for activating coagulation cascades in the event of a vascular breach.
The binding of FVII to its receptor, expressed by the cells of the subendothelium exposed by the lesion, allows its very rapid activation by traces of FXa, circulating in trace amounts in vivo.
The FT-FVIIa complex then causes the activation of FIX and FX and the formation of thrombin.
Upon relipidation, this product will promote clotting in a two-stage prothrombin time test.
Add 1.0 mL of filtered deionized or sterile water to generate a 25 μg/mL.
Store lyophilized vials at +2/+8°C. Store reconstituted protein in aliquots frozen at -20°C or colder, avoid freeze-thaw cycles.
The lyophilized presentation allows greater stability until the expiration date.
Screw capped clear glass vials of 25 μg of protein lyophilized from 10 mM TRIS HCI, 150 mM NaCI, 0.01% CHAPS, pH 8.0, with 200 mM mannitol.
