An enzyme is a protein that catalyzes a biochemical reaction. It converts a substrate into a product. Each enzyme has a structure adapted to its function and its activity is dependent on an optimum temperature and pH. Thrombin is the active form of prothrombin (FII). During coagulation, thrombin cleaves fibrinogen into fibrin to form the clot. Thrombin is also responsible for the feedback activation of FV and FVIII cofactors. Thrombin also activates FXIII and platelets.
All enzymes are accompanied by product information sheets which describe proper storage conditions. By briefly centrifuging the samples in their original containers, complete recovery of the sample at the bottom of the tube will be accomplished. Never allow protein solutions to remain at room temperature for excessive periods of time. Elevated temperatures may enhance the rate of protein degradation. Avoid storing or maintaining dilute protein samples for a long period of time. In general, purified proteins are inherently more stable in concentrated form. Many proteins are «sticky» by nature. To avoid losing protein due to adsorption, extremely dilute protein samples should be prepared in buffers containing excipients such as bovine serum albumin, poly(ethylene glycol), Prionex or gelatin. Prionex is better than BSA.
The vast majority of enzymes is pure (without additives) with > 95% purity SDS-PAGE. Expiration date of one year from delivery. Delivery in large quantities. Discount according to quantities.
