VON WILLEBRAND FACTOR
VWF ANTIGEN
ELISA ASSAY

TECHNOZYM® VWF:CBA ELISA

Cat Num. ConditioningPackage size  
4-5450301kit12 x 8
ELISA for determination of von Willebrand Factor collagen binding activity. In combination of von Willebrand disease type 1, 2 and 3 is possible.
In order to analyze the adhesive properties of the von Willebrand Factor, as a rule, the platelet aggregation is measured (measuring system = ristocetin-dependent platelet aggregation). However, this does not reflect the physiological setting nor the function of the vWF. For determining the adhesive properties of the vWF, its binding capacity to collagen serves as a parameter which corresponds to the physiological function of the vWF.

Kit contents:

  • 1 bottle of Washing buffer concentrate (100 mL)
  • 1 bottle of Incubation buffer(100 mL)
  • 5 calibrators, lyophilized
  • 1 vial of Low control plasma, lyophilized
  • 1 vial of High control plasma, lyophilized
  • 1 vial of conjugate (0.3 mL)
  • 1 bottle of TMB (12 mL)
  • 1 bottle of Stop Solution (12 mL)
  • 12 ELISA strips (12 x 8 wells)
  • 2 adhesive films
Features & benefits
  • Reagents are stable 6 months after opening
  • Normal range for vWF:CBA is between 0.6 – 1.3 U/mL (60 – 130 %)
  • Test time: 60 minutes
Characteristics
The von Willebrand Factor (vWF) is a large, multifunctional glycoprotein, occupying a key position in primary haemostasis. It has a multiple structure with several functions:
  • It is the carrier protein for Factor VIII in plasma; it forms a complex and thus protects Factor VIII from early proteolytic decomposition.
  • It acts as a mediator for platelet aggregation by attaching itself to platelet membrane receptors (GP Ib and GP lIb/IIIa) following previous platelet activation.
  • It plays a part in primary haemostasis by acting as a mediator between adhesioned platelets and the subendothelium (lesioned vascular wall).
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