TECHNOZYM® T-PA COMBI ACTIBIND® ELISA KIT
||Conditioning||Package size ||
|4-TC16000||kit||12 x 8 ||
A high sensitive ELISA for the combined quantitative determination of concentration and activity of t-PA (antigen and activity) in human plasma.
In the Actibind t-PA test an antibody which does not interfere with t-PA functional activity is coated onto a microtitre plate and used to bind t-PA contained in plasma to the plate surface. Following an incubation period, non-bound plasma components are washed away and an activity substrate solution containing Glu-plasminogen, CNBr-fragments of fibrinogen and a chromogenic plasmin substrate is incubated in the plate. The bound t-PA activates Glu-plasminogen to yield plasmin. The reaction between plasmin and the chromogenic plasmin substrate releases a coloured product whose concentration is proportional to the amount of active t-PA in the test sample. After photometrically measuring this reaction, the microtitre plate is washed to remove the activity substrate solution.The t-PA antigen remains bound to the plate. A horseradish peroxidase (POX) conjugated monoclonal anti-t-PA antibody which recognizes both active, t-PA and inactive t-PA is then incubated on the plate.
- 1 vial of calibrator, recombinant t-PA
- 1 vial of Conjugate (0.3 mL)
- 1 bottle of Incubation buffer (90 mL)
- 1 bottle of TMB (12 mL)
- 1 bottle of Stop Solution (15 mL)
- 1 bottle of wash solution (80 mL)
- 1 vial of Plasmine Activator mixture
- 1 bottle of Diluent (20 mL)
- 12 ELISA test strips (12 x 8 wells)
Tissue plasminogen activator (t-PA) and urokinase are the agents that convert plasminogen to the active plasmin, thus allowing fibrinolysis to occur. t-PA is released into the blood very slowly by the damaged endothelium of the blood vessels, such that, after several days (when the bleeding has stopped), the clot is broken down. This occurs because plasminogen became entrapped within the clot when it formed; as it is slowly activated, it breaks down the fibrin mesh.