FACTOR ASSAYS
FACTOR XIII
CHROMOGENIC AND ELISA ASSAYS

TECHNOZYM® FXIII AG ELISA

Cat Num. ConditioningPackage size  
4-5360100kit12 x 8
Antigene ELISA test used for determination of blood coagulation factor XIII (FXIII) in human plasma.
The high sensitivity of the assay allows the quantitative measurement of the extremely low plasma Factor XIII (FXIII) levels that might occur in congenital FXIII deficiency. Only the FXIII complex is measured, the presence of free subunits does not interfere with the assay. The TECHNOZYM® FXIII Ag ELISA kit is a one-step sandwich enzyme immunoassay using monoclonal antibodies specific to FXIII-A and FXIII-B subunits. Plasma FXIII is a heterotetramer (FXIII A2B2) consisting of A (FXIII-A) and B (FXIII-B) subunits. With the exception of the extremely rare inherited FXIII-B deficiency, all A subunits are complexed with B subunits in plasma, while about 50 % of the B subunits exist in free form.

Kit contents:

  • 12 ELISA test strips (12 x 8 wells)
  • 1 vial of Capture antibody
  • 1 bottle of Wash solution, concentrate (100 mL)
  • 1 bottle of Sample Dilution buffer (100 mL)
  • Calibrators, lyophilised; 1 vial each (0.25 mL)
  • Control plasmas high and low level; 1 vial each (0.25mL)
  • 2 bottles of TMB (12 mL)
  • 1 bottle of Stop solution (12 mL)
  • Adhesive film, 2 pieces.
Information
Coagulation factor XIII is a protransglutaminase that has a major role in the final stage of blood coagulation. The zymogen plasma FXIII (pFXIII) circulates in plasma as a tetramer (FXIII-A2B2) consisting of two catalytic A subunits (FXIII-A) and two carrier/protec-tive B subunits (FXIII-B). FXIII-A is produced by cells of bone marrow origin, whereas FXIII-B is synthesized by hepatocytes and forms complexes in the plasma.

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