FIBRINOLYSIS
FIBRONECTIN — VITRONECTIN
ELISA ASSAY

TECHNOZYM® FIBRONECTIN ELISA KIT

Cat Num. ConditioningPackage size  
4-TC12030kit12 x 8
A highly sensitive complete sandwich ELISA for the selective determination of intact fibronectin in human plasma.
The Technozym Fibronectin Test is used to measure human plasma fibronectin levels, particularly in patients with severe trauma, shock, sepsis, hepatic disease or clotting disorders. The Technozym Fibronectin Test is a solid phase enzyme immunoassay. The peroxidase conjugated anti-fibronectin monoclonal antibody used in this kit recognises only intact fibronectin, i.e. split products of the molecule are not detected. In normal plasma, approximately two - thirds of the total quantity of circulating fibronectin is in intact form. In patients with sever septicemia or disseminated intravascular coagulation (DIC) fibronectin split products are generated by the action of leucocyte elastase or plasmin on the intact molecule leading to a rapid decrease in the levels of functionally intact fibronectin. The normal plasma concentration of total fibronectin is 330 + 80 μg/mL, and native uncleaved Fibronectin is 70 – 148 μg/mL.

Kit contents:

  • 1 bottle of Wash solution (80 mL)
  • 1 bottle of Incubation buffer (90 mL)
  • 1 calibrator plasma, lyophilized
  • 1 vial of concentrated Conjugate
  • 3 bottles of Dilution buffer (20 mL)
  • 1 bottle of TMB (12 mL)
  • 1 bottle of Stop solution (15 mL)
  • 12 ELISA test strips (12 x 8 wells)
  • 2 adhesive films
Use
  • Test time: 120 minutes
  • Sensitivity: 0 - 2 µg/mL of fibronectin

M.S.D.S.

4-TC12030

Notice

4-TC12030

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