FACTOR ASSAYS
FACTOR XIII
CHROMOGENIC AND ELISA ASSAYS

TECHNOCHROM® FXIII

Cat Num. ConditioningPackage size  
4-5360010kit3 x 20
Reagent kit for the determination of blood coagulation Factor XIII (FXIII) activity to detect inherited or acquired FXIII deficiencies, abnormal FXIII with decreased activity and elevated FXIII level.
FXIII present in the plasma sample is activated by thrombin and Ca²+. The formed FXIIIa then cross-links the amine substrate glycine ethyl ester (GEE) to the glutamine residue of specific peptide substrate PI(1-12), and ammonia is released. In the indicator reaction the amount of released ammonia is monitored in a glutamate dehydrogenase catalysed NADPH-dependent reaction. The consumption of NADPH is measured spectrophotometrically by the decrease of absorbance at 340 nm. Within a time window the decrease of absorbance is directly proportional to the FXIII activity. This assay can be used for monitoring FXIII replacement therapy.

Kit contents:

  • 3 vials of Activator Reagent (3 mL)
  • 3 vials of Detection Reagent (3 mL)
  • 3 vials of NADPH Solution (3 mL)
  • 3 vials of Inhibitor Reagent (1 mL)
  • 1 vial of Stabilizer Solution (6 mL)
Information
Inherited FXIII deficiency is a rare, but occurs with severe bleeding diathesis with occasional wound healing impairment and in women with habitual abortion.
Advantages
The method is linear up to 300 % FXIII activity. The detection limit is 0.6 % with blank reagent included. "When a blank reagent is not supplied by the manufacturer, the limit of quantification is between 3 % and 5 %." H. P. KOHLER and al, Diagnosis and classification of factor XIII deficiencies, journal of Thrombosis and Haemostasis, 9: 1404–1406 July 2011 Lawrie et al, J Thrombosis and haemostasis 2010;8: 2478-82

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