FACTOR ASSAYS
FACTOR XIa
CHROMOGENIC ASSAY

ROX FACTOR XIA

Cat Num. ConditioningPackage size  
5-110050kit2 x 50
A chromogenic kit for quantitative activity determination of Factor XIa in enriched or highly purified protein preparations.
Human FXIa is determined from its activation of human Factor IX and ensuing activation of human Factor X. Generated Factor Xa is then measured with a chromogenic FXa substrate. The activation of Factor IX is performed in two steps: Initial activation of FIX in the absence of phospholipids and continued activation of FIX in the presence of FX and phospholipids, therewith allowing concomitant activation of FX.

Kit contents:

  • Reagent 1 (2 vials) – REF 1110
  • Reagent 2 (2 vials) – REF 1120
  • FXa Substrate, 6 mL (1 vial) – REF 9080
  • Diluent Buffer, Stock Solution, 20 mL (1 vial) – REF 1150
Features & benefits
  • Detection Limit = about 0.03 mU/mL
  • No use of human plasma
Characteristics
Factor XI (FXI) is a dimeric protein of 160 kDa, which is activated to FXIa by FXIIa or by thrombin. Activation of FXI may occur inadvertently during protein purifications and FXIa can be a contaminant in intermediate or final products. FXIa functional activity is determined in a chromogenic method. FXIa in the sample activates human FIX to FIXa in the presence of calcium ions. Generated FIXa activates human FX in the presence of human FVIII, calcium ions and phospholipid. The amount of activated FX is determined from the hydrolysis of a chromogenic FXa substrate and is related to the FXIa activity of the sample. The concentration of functionally active FXIa is assigned vs. a FXIa Calibrator and expressed in Units. Not intended for analysis of patient plasma.

M.S.D.S.

5-110050

Notice

5-110050

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