FACTOR ASSAYS
FACTOR IX
CHROMOGENIC ASSAY

ROX FACTOR IX

Cat Num. ConditioningPackage size  
5-900020kit2 x 50
Kit for determination of Factor IX activity in plasma and Factor IX preparations, including potency assignment of FIX concentrates.
FIX is activated by human FXIa with concomitant activation of human FX by generated FIXa in the presence of FVIII, calcium ions and phospholipids. The amount of formed FXa is related to the FIX activity and is measured through hydrolysis of a chromogenic FXa substrate. There is no use of FIX deficiency plasma. The Kit contains a fibrin polymerization inhibitor and a heparin antagonist.

Kit contents:

  • 2 vials of Reagent A (Human Factors VIII and X, bovin Factor V, and a fibrin polymerization inhibitor).
  • 2 vials of Reagent B (Human Factors XIa and II, calcium chloride and phospholipids)
  • 1 vial of Chromogenic FXa Substrate, 6 mL
  • 1 bottle of Dilution buffer, 20 mL
Features & Benefits
  • There is no interference of FIXa up to 50 mIU FIXa/1 IU FIX.
  • No use of FIX deficiency plasma
  • Detection Limit: 0.1 % FIX (CLSI EP17-A)
  • Quantification Limit: 0.5 % FIX (CLSI EP17-A)
  • Linearity: 0.5 – 200 % (CLSI EP06-A)
Characteristics
FIX activity is determined in a chromogenic method, in which human FIX is activated by human FXIa and where formed FIXa activates human FX in the presence of human FVIII, calcium ions and phospholipid. Similar to in vivo conditions, FVIII is activated by thrombin which is generated during the incubation. The amount of FXa formed is related to the FIX activity and is determined from the hydrolysis of a chromogenic FXa substrate. The FIX activity of the sample is assigned vs. a FIX plasma or a FIX concentrate standard with FIX potency expressed in International Units (IU).

M.S.D.S.

5-9000-20

Notice

5-900020

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