RABBIT ELISA KITS

Cat Num.ProductSize
6-E-0001-RABBIT
Rabbit tissue-type plasiminogen actilyse, t-PA ELISA Kit96 tests
6-E-0014-RABBIT
Rabbit thrombin-antithrombin ,TAT ELISA Kit96 tests
6-E-0050-RABBIT
Rabbit coagulation factor II, FII ELISA Kit 96 tests
6-E-0065-RABBIT
Rabbit D-Dimer, DD ELISA Kit96 tests
6-E-0077-RABBIT
Rabbit fibrinogen degradation product, FDP ELISA Kit 96 tests
6-E-0078-RABBIT
Rabbit fibrinogen, Fbg ELISA Kit96 tests
6-E-0164-RABBIT
Rabbit plasmin-antiplasmin complex, PAP ELISA Kit96 tests
6-E-0165-RABBIT
Rabbit plasminogen activator inhibitor 1, PAI-1 ELISA Kit 96 tests
6-E-0166-RABBIT
Rabbit plasminogen activator inhibitor, PAI ELISA Kit 96 tests
6-E-0167-RABBIT
Rabbit plasminogen, Plg ELISA Kit96 tests
6-E-0168-RABBIT
Rabbit platelet activating factor, PAF ELISA Kit 96 tests
6-E-0169-RABBIT
Rabbit platelet factor 4, PF-4 ELISA Kit96 tests
6-E-0170-RABBIT
Rabbit platelet-derived growth factor, PDGF ELISA Kit 96 tests
6-E-0180-RABBIT
Rabbit prothrombin fragment 1+2, F1+2 ELISA Kit 96 tests
6-E-0201-RABBIT
Rabbit thrombin activatable fibrinolysis inhibitor, TAFI ELISA Kit96 tests
6-E-0202-RABBIT
Rabbit thrombin receptor, TR ELISA Kit 96 tests
6-E-0203-RABBIT
Rabbit thrombomodulin, TM ELISA Kit 96 tests
6-E-0204-RABBIT
Rabbit thromboxane B2, TX-B2 ELISA Kit96 tests
6-E-0207-RABBIT
Rabbit tissue factor pathway inhibitor, TFPI ELISA Kit96 tests
6-E-0208-RABBIT
Rabbit tissue factor, TF ELISA Kit96 tests
6-E-0209-RABBIT
Rabbit tissue factor, TF ELISA Kit96 tests
6-E-0213-RABBIT
Rabbit tissue-type plasminogen actilyse, t-PA ELISA Kit96 tests
6-E-0220-RABBIT
Rabbit urokinase plasminogen activator, uPA ELISA kit 96 tests
6-E-0221-RABBIT
Rabbit urokinase-type plasminogen activator receptor, PLAUR/uPAR ELISA kit 96 tests
6-E-0222-RABBIT
Rabbit vascular endothelial cell growth factor, VEGF ELISA Kit96 tests
6-E-0223-RABBIT
Rabbit vascuolar cell adhesion molecule 1, VCAM-1 ELISA kit96 tests
6-E-0224-RABBIT
Rabbit vasoactive intestinal peptide, VIP ELISA Kit96 tests
6-E-0225-RABBIT
Rabbit von Willebrand Factor, VWF ELISA Kit96 tests
6-E-0226-RABBIT
Rabbit α2-antiplasmin, α2-AP ELISA Kit96 tests
6-E-0227-RABBIT
Rabbit β-thromboglobulin, β-TG ELISA Kit96 tests

Description


The recommendations below represent general guidelines and are not meant to be absolutes. Optimization of assay conditions is the responsibility of the researcher.
Basic buffers:
  • TBS buffer: 8.76 grams of NaCl and 2.42 grams of Tris base in 900 mL of deionized water. Adjust pH to 7.4 with 6N HCl. Adjust the volume to 1 liter with deionized water.

  • Coating buffer: 50 mM Sodium Carbonate, pH 9.6. 1.7 grams of Na₂CO₃, 2.86 grams of NaHCO₃ in 900 mL of deionized water. Check the pH to assure that it is 9.6 and adjust if necessary. Adjust the final volume to 1 liter using deionized water.

  • Blocking buffer: TBS containing 2.0 % (w/v) BSA.

  • Wash buffer: TBS containing 0.05 % (v/v) Tween 20

  • Assay buffer: TBS containing 0.02 %(w/v) BSA and 0.05% (v/v) Tween 20.

Coating plates: Select assay plates that have been certified for use in ELISA applications. Dilute the coating protein (generally an antibody, however some competitive assay formats may require the antigen to be immobilized on the plate) to the desired concentration in coating buffer. For monoclonal antibodies and affinity purified polyclonal antibodies, we recommend a coating concentration of 10 micrograms per mL. For non-affinity purified polyclonals, we recommend a coating concentration of 25 micrograms per mL. Add the coating solution to the assay plate (0.1 mL per well is standard practice), cover the plate and allow it to incubate for 2 hours at room temperature, or overnight (approximately 16 hours) at 4° C.
Blocking plates: Remove the coating solution from the plate and fill the wells to 90 % of their capacity (approximately 400 microliters for a standard 96 well plate) with blocking buffer. Incubate the plate for 2 hours at room temperature, or overnight (approximately 16 hours) at 4° C.
Standards: Standard curves are typically generated by diluting the standard to 100 nanograms per mL in assay buffer, and then serially diluting to make eight standard concentrations in the range of 100 to 0.78 nanograms per mL. Apply 0.1 mls of each dilution to appropriate wells in the plate. The standard is typically run in duplicate.
Samples: Samples are diluted in assay buffer so that they will fall within the working range of the standard curve (0.78 to 100 nanograms per mL). Apply 0.1 mls of each sample to the appropriate wells in the plate. The samples are typically run in duplicate at two or more dilutions.
Incubation times: Primary incubations (i.e., those involving application of the standard or samples to the plate) are generally performed for either 2 hours at room temperature or overnight (16 hours) at 4° C. Secondary incubations those involving the application of secondary antibodies or antibody conjugates are generally done for 1 hour at room temperature. Incubation times may be further shortened by performing steps at 37° C when possible. A dilution of 5 micrograms per mL for monoclonals and 10 micrograms per mL for polyclonals is recommended as a starting point. Further optimization may be necessary depending on your assay.
Development: The two most common enzymes utilized for detection are horseradish peroxidase and alkaline phosphatase. For horseradish peroxidase the most common substrates are tetramethylbenzidine (TMB) and o-phenylenediamine (OPD). The common substrate for alkaline phosphatase is p-nitrophenyl phosphate (PNPP). We recommend following the manufacturers instructions for the use of these substrates.
Optimization: Developing a sensitive and reproducible ELISA requires optimization of many conditions including choice of antibodies, antibody concentrations, incubation times, incubation temperatures, wash conditions, etc. The choice of antibody and the concentration of antibody used may affect both sensitivity and background noise. In addition, more (or less) stringent buffer conditions may be required to achieve optimal results.

THEY TRUST US

OUR PARTNERS