||Conditioning||Package size ||
|8-800186||kit||2 x 40 ||
Plasma based chromogenic assay for determination of Thrombin Activatable Fibrinolysis Inhibitor enzyme activity (TAFI)
Functional chromogenic assay based on direct substrate cleavage. TAFI, present in the plasma sample is activated by thrombin/thrombomodulin. A synthetic substrate is selectively and irreversibly cleaved by TAFIa, producing a thiol derivative. This thiol reacts with the colourless Ellman’s reagent splitting off the yellow coloured 5-mercapto-2-nitro-benzoic acid. The extinction at wavelength 405 nm measured at the end of the enzymatic reaction is directly proportional to the concentration of TAFI activated by thrombin/thrombomodulin.
- 2 vials of Activator (4 mL)
- 2 vials of Start Reagent (4 mL)
- 2 vials of Diluent (5 mL)
- Calibrators and controls included