THROMBOPHILIA
TAFI
CHROMOGENIC ASSAY

PEFAKIT® TAFI

Cat Num. ConditioningPackage size  
8-800186kit2 x 40
Plasma based chromogenic assay for determination of Thrombin Activatable Fibrinolysis Inhibitor enzyme activity (TAFI)
Functional chromogenic assay based on direct substrate cleavage. TAFI, present in the plasma sample is activated by thrombin/thrombomodulin. A synthetic substrate is selectively and irreversibly cleaved by TAFIa, producing a thiol derivative. This thiol reacts with the colourless Ellman’s reagent splitting off the yellow coloured 5-mercapto-2-nitro-benzoic acid. The extinction at wavelength 405 nm measured at the end of the enzymatic reaction is directly proportional to the concentration of TAFI activated by thrombin/thrombomodulin.

Kit contents:

  • 2 vials of Activator (4 mL)
  • 2 vials of Start Reagent (4 mL)
  • 2 vials of Diluent (5 mL)
  • Calibrators and controls included
Use
The test can be run on fully automated coagulation analyzers by single determinations, giving results within 5 minutes.
Features & benefits
  • Functional assay based on direct substrate cleavage
  • Specific for TAFI
  • Stable reagents
Characteristics
Characteristics:
Performance either on fully automated coagulation analyzers or microtiterplate readers:
  • Single determinations
  • Easy handling
  • Results in 3 minutes
  • No additional equipment needed
  • Validated protocols

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