NATURAL PROTEASE INHIBITORS

Cat Num.ProductActivityPM (g/mol)FormulationSize
9-HCII-0190

Heparin Cofactor II

Inhibits thrombin, alpha-chymotrypsin, Cathepsin G, protease B, Streptomyces griseus

700 to 1 800 units/mg65 60050/50 (v/v) glycerol/H₂O; 5 mM calcium chloride100 µg
9-HCATIII-0120

Human Antithrombin

Inactivates several serine proteinases

0.7 to 1.0 mole thrombin / mole AT58 00050/50 (v/v) glycerol/H₂O; 5 mM calcium chloride1 mg
6-ATIII-10

Human Antithrombin

Inactivates several serine proteinases

10 UI58 000tampon/NaCl1.5 mg
9-MCATIII-5120

Mouse Antithrombin

Inactivates several serine proteinases

0.6 to 1.0 mole thrombin / mole AT58 00050/50 (v/v) glycerol/H₂O; 5 mM calcium chloride100 µg
9-CTI-01

Corn Trypsin Inhibitor

CTI is extracted from ground kernels of corn into a physiologic buffer. Preparations of CTI are tested for the ability to prolong the aPTT assay without affecting the PT assay. The specific activity of each lot of CTI is determined, and one unit is defined as the amount of CTI required to double the aPTT of normal human plasma. CTI inhibits FXIIa and trypsin.

12 50020 mM Tris; 150 mM NaCl;
pH 7.4, < -70° C
1 mg
9-TAFI-01

Thrombin Activatable Fibrinolysis Inhibitor

After thrombin activation, the mature protein downregulates fibrinolysis.

2.0 to 9.2 units/mg60 00020 mM HEPES; 150 mM NaCl; pH 7.450 µg
9-ANG-01

Human Angiostatin

Inhibits the proliferation of endothelial cells

≈ 50 00020 mM HEPES; 0.15 M NaCl ; pH 7.4500 µg
9-HA2AP-0230

α2-Plasmin Inhibitor

Physiological inhibitor of plasmin, forming an irreversible complex in its catalytic site; prevents binding of plasmin to fibrin.

67 00050 mM phosphate potassium; 7.5 mM KCl; 7.575 mM EDTA; pH 7.4100 µg
8-800277

Aprotinin

Aprotinin is a polyvalent reversible inhibitor of serine proteinases. Aprotinin Pentapharm can be used for the isolation of proteins as well as for biopharmaceutical downstream purification to inhibit undesired proteolytic activity of serine proteases such as trypsin, plasmin, trypsinogen, urokinase, chymotrypsin, kallikrein, elastase and others. It can also be used during immunodiffusion, radioimmunoassay or enzyme-linked immuno assay procedures. Aprotinin is used in chromogenic assays for the determination of antithrombin III, heparin, α2-macroglobulin, factor Xa and thrombin to inhibit disturbing kallikrein or plasmin activities.

Aprotinine lyophilized (1g/vial)6 512≈ 6 M KIU/vial1 g
8-073-70

Aprotinin

Aprotinine solution (≈ 200 000 KIU/ml)6512M KIU
8-126-05

R-Hirudin

This recombinant protein is the most potent and specific thrombin inhibitor known.

6963.52 000 ATU
6-H7035-P01

TFPI recombinant

Inhibits the factor VIIa and tissue factor in the complexe Xa/TFPI/FVIIa/TF, pH 8,0

0.04 µg/µl53 35050 mM Tris-HCl; 10 mM glutathion50 µg
9-HCPZ-0220

Human Protein Z

The main role of protein Z appears to be the degradation of factor Xa. This is done by protein Z-related protease inhibitor (ZPI), but the reaction is accelerated 1000-fold by the presence of protein Z, phospholipids and Ca²+.

62 00050/50 (v/v) glycerol/H₂O; 5 mM CaCl₂100 µg


Inhibition constants Ki in μM of Pefabloc inhibitors
for serine proteinases

Description


Detection and determination of proteinases, studies on their interactions with substrates and effectors and the investigation of their physiological role are greatly facilitated by the use of proteinase inhibitors.
In this context, especially synthetic, low-molecular weight inhibitors of different selectivity are very useful. They are widely applied during purification and characterization of proteinases.
Furthermore, synthetic inhibitors are useful tools for suppression of undesired proteolytic activity. Depending upon the manner in which the inhibitor is attached to the enzyme, one distinguishes reversible and irreversible inhibitors.

Characteristics


Pentapharm offers a selection of inhibitors derived from benzamidine. They can help in the characterization of trypsin-like enzymes.
Most Pefablocs® exhibit a selective inhibitory activity on certain trypsin-like proteinases of physiological relevance. However, each inhibitor will of course display a characteristic action on other serine proteinases (see table on Ki-values page 47).

Advantages


Supplied lyophilized or frozen
Expiry date > 1 year
Glass vial or plastic tubes
Discount according to quantities

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