Cat Num. Product Size
Mouse activated protein C, APC ELISA Kit 96 tests
Mouse anti-cardiolipin antibody IgA, ACA-IgA ELISA Kit 96 tests
Mouse anti-cardiolipin antibody IgG, ACA-IgG ELISA Kit 96 tests
Mouse anti-cardiolipin antibody IgM, ACA-IgM ELISA Kit 96 tests
Mouse anti-thrombin receptor, ATR ELISA Kit 96 tests
Mouse Anti-trypsin, AT ELISA Kit 96 tests
Mouse coagulation factor II, FII ELISA Kit 96 tests
Mouse coagulation factor VIII related antigen, FVIII-Ag ELISA Kit 96 tests
Mouse coagulation factor X, FX ELISA Kit 96 tests
Mouse factor VIII-related antigen, FVIIIAg ELISA Kit 96 tests
Mouse coagulation factor IX, FIX ELISA Kit 96 tests
Mouse fibrinogen, Fbg ELISA Kit 96 tests
Mouse phosphatidylinositol antibody IgG/IgM, PI Ab-IgG/IgM ELISA Kit 96 tests
Mouse phosphatidylserine, PS ELISA Kit 96 tests
Mouse phosphatidylserine, PS ELISA Kit 96 tests
Mouse platelet activating factor, PAF ELISA KIT 96 tests
Mouse platelet antibodies IgG/M/A, PA-IgG/M/A ELISA Kit 96 tests
Mouse platelet factor 3, PF3 ELISA Kit 96 tests
Mouse platelet membrane glycoprotein IIbIIIa, GP-IIbIIIa ELISA Kit 96 tests
Mouse platelet-derived growth factor AB, PDGF-AB ELISA kit 96 tests
Mouse thrombin receptor, TR ELISA Kit 96 tests
Mouse thrombin-antithrombin complex, TAT ELISA Kit 96 tests
Mouse thrombospondin 1, TSP-1 ELISA Kit 96 tests
Mouse thromboxane B2, TXB2 ELISA Kit 96 tests
Mouse thrombus precursor protein, TpP ELISA Kit 96 tests
Mouse von Willebrand Factor, vWF ELISA Kit 96 tests
Mouse α1-microglobulin, α1-MG ELISA Kit 96 tests
Mouse α2-Antiplasmin, α2-AP ELISA Kit 96 tests
Mouse α2-plasmin inhititor, α2-PI ELISA Kit 96 tests
Mouse fibrinogen (Fb) ELISA Kit. 96 tests
Mouse thromboxane A2 (TXA2) ELISA Kit. 96 tests
Mouse thrombomodulin (TM) ELISA Kit. 96 tests
Mouse tissue type plasminogen activator (T-PA) ELISA Kit. 96 tests
Mouse tissue factor pathway inhibitor (TFPI) ELISA Kit. 96 tests


The recommendations below represent general guidelines and are not meant to be absolutes. Optimization of assay conditions is the responsibility of the researcher.
Basic buffers:
  • TBS buffer: 8.76 grams of NaCl and 2.42 grams of Tris base in 900 mL of deionized water. Adjust pH to 7.4 with 6N HCl. Adjust the volume to 1 liter with deionized water.

  • Coating buffer: 50 mM Sodium Carbonate, pH 9.6. 1.7 grams of Na₂CO₃, 2.86 grams of NaHCO₃ in 900 mL of deionized water. Check the pH to assure that it is 9.6 and adjust if necessary. Adjust the final volume to 1 liter using deionized water.

  • Blocking buffer: TBS containing 2.0 % (w/v) BSA.

  • Wash buffer: TBS containing 0.05 % (v/v) Tween 20

  • Assay buffer: TBS containing 0.02 %(w/v) BSA and 0.05% (v/v) Tween 20.

Coating plates: Select assay plates that have been certified for use in ELISA applications. Dilute the coating protein (generally an antibody, however some competitive assay formats may require the antigen to be immobilized on the plate) to the desired concentration in coating buffer. For monoclonal antibodies and affinity purified polyclonal antibodies, we recommend a coating concentration of 10 micrograms per mL. For non-affinity purified polyclonals, we recommend a coating concentration of 25 micrograms per mL. Add the coating solution to the assay plate (0.1 mL per well is standard practice), cover the plate and allow it to incubate for 2 hours at room temperature, or overnight (approximately 16 hours) at 4° C.
Blocking plates: Remove the coating solution from the plate and fill the wells to 90 % of their capacity (approximately 400 microliters for a standard 96 well plate) with blocking buffer. Incubate the plate for 2 hours at room temperature, or overnight (approximately 16 hours) at 4° C.
Standards: Standard curves are typically generated by diluting the standard to 100 nanograms per mL in assay buffer, and then serially diluting to make eight standard concentrations in the range of 100 to 0.78 nanograms per mL. Apply 0.1 mls of each dilution to appropriate wells in the plate. The standard is typically run in duplicate.
Samples: Samples are diluted in assay buffer so that they will fall within the working range of the standard curve (0.78 to 100 nanograms per mL). Apply 0.1 mls of each sample to the appropriate wells in the plate. The samples are typically run in duplicate at two or more dilutions.
Incubation times: Primary incubations (i.e., those involving application of the standard or samples to the plate) are generally performed for either 2 hours at room temperature or overnight (16 hours) at 4° C. Secondary incubations those involving the application of secondary antibodies or antibody conjugates are generally done for 1 hour at room temperature. Incubation times may be further shortened by performing steps at 37° C when possible. A dilution of 5 micrograms per mL for monoclonals and 10 micrograms per mL for polyclonals is recommended as a starting point. Further optimization may be necessary depending on your assay.
Development: The two most common enzymes utilized for detection are horseradish peroxidase and alkaline phosphatase. For horseradish peroxidase the most common substrates are tetramethylbenzidine (TMB) and o-phenylenediamine (OPD). The common substrate for alkaline phosphatase is p-nitrophenyl phosphate (PNPP). We recommend following the manufacturers instructions for the use of these substrates.
Optimization: Developing a sensitive and reproducible ELISA requires optimization of many conditions including choice of antibodies, antibody concentrations, incubation times, incubation temperatures, wash conditions, etc. The choice of antibody and the concentration of antibody used may affect both sensitivity and background noise. In addition, more (or less) stringent buffer conditions may be required to achieve optimal results.