HUMAN ELISA KITS

Cat Num.ProductSize
4-E-0041HUM
Human abnormal prothrombin, APT ELISA Kit96 tests
4-E-0050HUM
Human activated coagulation factor XII, FXIIa ELISA Kit96 tests
4-E-0052HUM
Human activated protein C resistance, APCR ELISA Kit96 tests
4-E-0053HUM
Human activated protein C, APC ELISA Kit96 tests
4-E-0130HUM
Human annexin V, ANX-V ELISA Kit 96 tests
4-E-0131HUM
Human annexin-V ELISA Kit96 tests
4-E-0144HUM
Human anti-cardiolipin antibody IgA, ACA-IgA ELISA Kit96 tests
4-E-0145HUM
Human anti-cardiolipin antibody IgG, ACA-IgG ELISA Kit96 tests
4-E-0146HUM
Human anti-cardiolipin antibody IgM, ACA-IgM ELISA Kit96 tests
4-E-0182HUM
Human anti-glucoprotein 210, GP210 ELISA Kit96 tests
4-E-0183HUM
Human anti-glycoprotein antibody, GP ELISA Kit96 tests
4-E-0234HUM
Human anti-phosphatidyl serine antibody, APSA ELISA Kit96 tests
4-E-0235HUM
Human anti-phospholipid antibody, Apl/APA ELISA Kit96 tests
4-E-0263HUM
Human anti-thrombin III, AT-III ELISA Kit96 tests
4-E-0264HUM
Human anti-thrombin receptor, ATR ELISA Kit96 tests
4-E-0273HUM
Human anti-trypsin, AT ELISA Kit96 tests
4-E-0469HUM
Human coagulation factor II, FII ELISA Kit96 tests
4-E-0470HUM
Human coagulation tissular factor TF ELISA Kit96 tests
4-E-0471HUM
Human coagulation factor VII, FVII ELISA Kit96 tests
4-E-0472HUM
Human coagulation factor VIII related antigen, FVIII-Ag ELISA Kit96 tests
4-E-0473HUM
Human coagulation factor IX, FIX ELISA Kit96 tests
4-E-0474HUM
Human coagulation factor X, FX ELISA Kit96 tests
4-E-0475HUM
Human coagulation factor XI, FXI ELISA Kit96 tests
4-E-0476HUM
Human coagulation factor XII, FXII ELISA Kit96 tests
4-E-0477HUM
Human coagulation factor XIII, FXIII ELISA kit96 tests
4-E-0598HUM
Human D-Dimer, D2D ELISA Kit96 tests
4-E-0610HUM
Human dermatan sulfate, DS ELISA Kit96 tests
4-E-0614HUM
Human desmopressin/1-desamino-8-D-arginine vasopressin, dDAVP ELISA Kit96 tests
4-E-0640HUM
Human elastase ELISA Kit96 tests
4-E-0705HUM
Human fibrin ELISA Kit96 tests
4-E-0706HUM
Human fibrinogen degradation product, FDP ELISA Kit96 tests
4-E-0707HUM
Human fibrinogen like peptide 2, fgl2 ELISA Kit96 tests
4-E-0708HUM
Human fibrinogen, Fbg ELISA Kit96 tests
4-E-0709HUM
Human fibrinopeptide A, FPA ELISA Kit96 tests
4-E-0710HUM
Human fibrinopeptide B, FPB ELISA Kit96 tests
4-E-0713HUM
Human fibronectin, FN ELISA Kit 96 tests
4-E-0714HUM
Human fibronectin, FN ELISA Kit 96 tests
4-E-0715HUM
Human fibronectin, FN ELISA Kit 96 tests
4-E-0847HUM
Human heparan sulfate, HS ELISA Kit96 tests
4-E-0848HUM
Human heparanase, HPA ELISA kit96 tests
4-E-0849HUM
Human heparin associated antibody, HIT ELISA Kit96 tests
4-E-0851HUM
Human heparin sulphate protoglycans, HSPG ELISA Kit96 tests
4-E-0852HUM
Human heparin-binding epidermal growth factor-like growth factor, HB-EGF ELISA Kit96 tests
4-E-0882HUM
Human hirudin, HRD ELISA Kit96 tests
4-E-1023HUM
Human kallikrein 1, KLK 1 ELISA Kit 96 tests
4-E-1024HUM
human kallikrein 10, hk10 ELISA kit96 tests
4-E-1025HUM
Human kallikrein 10, KLK 10 ELISA Kit 96 tests
4-E-1026HUM
Human kallikrein 11, KLK 11 ELISA Kit 96 tests
4-E-1027HUM
Human kallikrein 6, KLK 6 ELISA Kit96 tests
4-E-1096HUM
Human low molecular weight heparin, LMWH ELISA Kit96 tests
4-E-1101HUM
Human L-selectin ELISA kit 96 tests
4-E-1307HUM
Human osteocalcin/bone gla protein, OT/BGP ELISA kit96 tests
4-E-1308HUM
Human osteocalcin/bone gla protein, OT/BGP ELISA kit96 tests
4-E-1311HUM
Human osteonectin, ON ELISA Kit96 tests
4-E-1312HUM
Human osteopontin, OPN ELISA Kit96 tests
4-E-1313HUM
Human osteopontin, OPN ELISA Kit96 tests
4-E-1314HUM
Human osteoprotegerin, OPG ELISA KIT 96 tests
4-E-1401HUM
Human plasma anticoagulant protein C, PC ELISA Kit96 tests
4-E-1402HUM
Human plasma anticoagulant protein S, PS ELISA Kit96 tests
4-E-1403HUM
Human plasma fibronectin, pFN ELISA kit96 tests
4-E-1404HUM
Human plasmin/fibrinolysin, PL/Fbn ELISA Kit96 tests
4-E-1405HUM
Human plasmin-antiplasmin complex, PAP ELISA Kit96 tests
4-E-1406HUM
Human plasminogen activator inhibitor 1, PAI-1 Elisa Kit96 tests
4-E-1407HUM
Human plasminogen activator inhibitor 1, PAI-1 ELISA Kit 96 tests
4-E-1408HUM
Human plasminogen activator inhibitor, PAI ELISA Kit 96 tests
4-E-1409HUM
Human plasminogen activator, PLGA ELISA Kit96 tests
4-E-1410HUM
Human plasminogen, Plg ELISA kit96 tests
4-E-1411HUM
Human plasminogen, Plg ELISA Kit96 tests
4-E-1412HUM
Human platelet activating factor, PAF ELISA Kit96 tests
4-E-1413HUM
Human platelet antibodies IgG/M/A, PA-IgG/M/A ELISA Kit96 tests
4-E-1414HUM
Human platelet associated antibody, PA-IgM ELISA Kit96 tests
4-E-1415HUM
Human platelet basic protein, PBP ELISA Kit96 tests
4-E-1416HUM
Human platelet factor 3, PF3 ELISA Kit96 tests
4-E-1417HUM
Human platelet factor 4, PF-4 ELISA Kit96 tests
4-E-1418HUM
Human platelet membrane glycoprotein IIbIIIa, GP‑IibIIIa ELISA Kit96 tests
4-E-1419HUM
Human platelet membrane glycoprotein IV, GP-IV ELISA Kit96 tests
4-E-1420HUM
Human platelet-associated complement 3, PAC3 ELISA Kit96 tests
4-E-1421HUM
Human platelet-associated immunoglobulins, PAIg ELISA Kit96 tests
4-E-1422HUM
Human platelet-derived growth factor AB, PDGF-AB ELISA kit 96 tests
4-E-1423HUM
Human platelet-derived growth factor receptor α, PDGFR-α ELISA kit96 tests
4-E-1424HUM
Human platelet-derived growth factor soluble receptor α, PDGFsR-α ELISA kit 96 tests
4-E-1425HUM
Human platelet-derived growth factor, PDGF ELISA Kit 96 tests
4-E-1426HUM
Human platelet-derived growth factor-BB, PDGF-BB ELISA Kit 96 tests
4-E-1427HUM
Human platelet-derived growth factor-BB, PDGF-BB ELISA kit 96 tests
4-E-1471HUM
Human protein C ELISA Kit96 tests
4-E-1472HUM
Human protein C, PC ELISA kit96 tests
4-E-1473HUM
Human protein carbonyl, PC ELISA Kit 96 tests
4-E-1482HUM
Human protein S ELISA Kit96 tests
4-E-1486HUM
Human protein Z ELISA Kit96 tests
4-E-1493HUM
Human prothrombin fragment 1+2, F1+2 ELISA kit96 tests
4-E-1494HUM
Human prothrombin, PT ELISA Kit96 tests
4-E-1618HUM
Human soluble P-selectin, sP-selectin ELISA Kit96 tests
4-E-1681HUM
Human thrombin, TM ELISA Kit96 tests
4-E-1682HUM
Human thrombin-antithrombin complex, TAT ELISA Kit96 tests
4-E-1683HUM
Human thrombin-thrombomodulin compound, T-TM ELISA Kit96 tests
4-E-1684HUM
Human thrombomodulin, TM ELISA Kit 96 tests
4-E-1685HUM
Human thrombomodulin, TM ELISA Kit 96 tests
4-E-1686HUM
Human thrombopoietin, TPO ELISA kit 96 tests
4-E-1687HUM
Human thrombospondin 1, TSP-1 ELISA Kit96 tests
4-E-1688HUM
Human thromboxane A2, TX-A2 ELISA Kit96 tests
4-E-1689HUM
Human thromboxane B2, TX-B2 ELISA Kit96 tests
4-E-1690HUM
Human thrombus precursor protein, TpP ELISA Kit96 tests
4-E-1708HUM
Human tissue factor pathway inhibitor, TFPI ELISA Kit96 tests
4-E-1709HUM
Human tissue factor, TF ELISA Kit96 tests
4-E-1717HUM
Human tissue-type plasminogen actilyse, t-PA ELISA Kit96 tests
4-E-1718HUM
Human tissue-type plasminogen activator, t-PA ELISA kit96 tests
4-E-1728HUM
Human protein S total, TPS ELISA Kit96 tests
4-E-1797HUM
Human urokinase, UK ELISA Kit96 tests
4-E-1798HUM
Human urokinase-type plasminogen activator receptor, PLAUR/uPAR ELISA kit96 tests
4-E-1799HUM
Human urokinase-type plasminogen activator, uPA/PLAU ELISA Kit96 tests
4-E-1800HUM
Human urokinase-type plasminogen activator, uPA/PLAU ELISA Kit96 tests
4-E-1839HUM
Human von Willebrand Factor, vWF ELISA kit96 tests
4-E-1840HUM
Human von Willebrand Factor, vWF ELISA Kit96 tests
4-E-1847HUM
Human α1-antitrypsin, α1-AT ELISA Kit96 tests
4-E-1848HUM
Human α1-microglobulin, α1-MG ELISA Kit96 tests
4-E-1849HUM
Human α1β glycoprotein, α1β-GP ELISA Kit96 tests
4-E-1850HUM
Human α2 macroglobulin, α2 MG ELISA Kit96 tests
4-E-1851HUM
Human α2-antiplasmin, α2-AP ELISA Kit96 tests
4-E-1852HUM
Human α2-plasmin inhibitor, α2-PI ELISA Kit96 tests
4-E-1874HUM
Human β2-glycoprotein 1 antibody IgA, β2-GP1 Ab IgA ELISA Kit96 tests
4-E-1875HUM
Human β2-glycoprotein 1 antibody IgA/G/M, β2-GP1 IgA/G/M ELISA Kit96 tests
4-E-1876HUM
Human β2-glycoprotein 1 antibody IgG, β2-GP1 Ab IgG ELISA Kit96 tests
4-E-1877HUM
Human β2-glycoprotein 1 antibody IgM, β2-GP1 Ab IgM ELISA Kit96 tests
4-E-1886HUM
Human β-thromboglobulin, β-TG ELISA Kit96 tests
4-E-2014HUM
Human antitrypsin (AT)ELISA Kit96 tests

Description


The recommendations below represent general guidelines and are not meant to be absolutes. Optimization of assay conditions is the responsibility of the researcher.
Basic buffers:
  • TBS buffer: 8.76 grams of NaCl and 2.42 grams of Tris base in 900 mL of deionized water. Adjust pH to 7.4 with 6N HCl. Adjust the volume to 1 liter with deionized water.

  • Coating buffer: 50 mM Sodium Carbonate, pH 9.6. 1.7 grams of Na₂CO₃, 2.86 grams of NaHCO₃ in 900 mL of deionized water. Check the pH to assure that it is 9.6 and adjust if necessary. Adjust the final volume to 1 liter using deionized water.

  • Blocking buffer: TBS containing 2.0 % (w/v) BSA.

  • Wash buffer: TBS containing 0.05 % (v/v) Tween 20

  • Assay buffer: TBS containing 0.02 %(w/v) BSA and 0.05% (v/v) Tween 20.

Coating plates: Select assay plates that have been certified for use in ELISA applications. Dilute the coating protein (generally an antibody, however some competitive assay formats may require the antigen to be immobilized on the plate) to the desired concentration in coating buffer. For monoclonal antibodies and affinity purified polyclonal antibodies, we recommend a coating concentration of 10 micrograms per mL. For non-affinity purified polyclonals, we recommend a coating concentration of 25 micrograms per mL. Add the coating solution to the assay plate (0.1 mL per well is standard practice), cover the plate and allow it to incubate for 2 hours at room temperature, or overnight (approximately 16 hours) at 4° C.
Blocking plates: Remove the coating solution from the plate and fill the wells to 90 % of their capacity (approximately 400 microliters for a standard 96 well plate) with blocking buffer. Incubate the plate for 2 hours at room temperature, or overnight (approximately 16 hours) at 4° C.
Standards: Standard curves are typically generated by diluting the standard to 100 nanograms per mL in assay buffer, and then serially diluting to make eight standard concentrations in the range of 100 to 0.78 nanograms per mL. Apply 0.1 mls of each dilution to appropriate wells in the plate. The standard is typically run in duplicate.
Samples: Samples are diluted in assay buffer so that they will fall within the working range of the standard curve (0.78 to 100 nanograms per mL). Apply 0.1 mls of each sample to the appropriate wells in the plate. The samples are typically run in duplicate at two or more dilutions.
Incubation times: Primary incubations (i.e., those involving application of the standard or samples to the plate) are generally performed for either 2 hours at room temperature or overnight (16 hours) at 4° C. Secondary incubations those involving the application of secondary antibodies or antibody conjugates are generally done for 1 hour at room temperature. Incubation times may be further shortened by performing steps at 37° C when possible. A dilution of 5 micrograms per mL for monoclonals and 10 micrograms per mL for polyclonals is recommended as a starting point. Further optimization may be necessary depending on your assay.
Development: The two most common enzymes utilized for detection are horseradish peroxidase and alkaline phosphatase. For horseradish peroxidase the most common substrates are tetramethylbenzidine (TMB) and o-phenylenediamine (OPD). The common substrate for alkaline phosphatase is p-nitrophenyl phosphate (PNPP). We recommend following the manufacturers instructions for the use of these substrates.
Optimization: Developing a sensitive and reproducible ELISA requires optimization of many conditions including choice of antibodies, antibody concentrations, incubation times, incubation temperatures, wash conditions, etc. The choice of antibody and the concentration of antibody used may affect both sensitivity and background noise. In addition, more (or less) stringent buffer conditions may be required to achieve optimal results.

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