FLUOROGENIC AMC SUBSTRATE FOR tPA
|Cat Num.||Product||Sequence||MW(Da)||Km / Kcat||Size|
|CH₃SO₂-D-Phe-Gly-Arg-AMC, AcOH||673.8||0.14mM / 11 s-1||25 mg|
The MCAPEP® and PEFAFLUOR® line of fluorogenic peptide substrates is a line of high quality substrates which allow testing of serine proteinases.
Their focus is on enzymes involved in coagulation and fibrinolysis where the line of substrates carry all the traditional substrates for thrombin, factor Xa, factor XIIa, kallikrein, activated protein C, plasmin and plasminogen-SK.
Fluorogenic substrates are synthetic peptides that react with proteolytic enzymes under formation of fluorescence which can be followed spectrophotometrically and the intensity of which is proportional to the proteolytic activity of the enzyme.
Typically, such substrates are composed of 3 to 5 natural or artificial amino acids. They may be N-terminally protected to reduce undesired degradation by aminopeptidases. On their C-termini they are modified so that upon cleavage of the amide bond a fluorogenic group is released and produce a fluorescent signal. Detection depends on the type of leaving group and may range from the UV- to the visible region of light.
Most commonly used group is the fluorogenic 7-amino-4-methylcoumarin (AMC) with an excitation wavelength at 342 nm and an emission wavelength at 440 nm.
All fluorogenic substrates are stable when stored at 2° C to 8° C.
MCA free content < 0.1 %
Purity grade > 95 %
Expiry date > 1 year
Glass vials 25 mg or 10 µmol
Package Inserts, certificate of analysis supplied.
Material safety Data Sheet (MSDS) supplied.
Prolonged stability following reconstitution (> 3 months)
We can supply milligram to gram.
Discount according to quantities