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The CHROMOPEP PC kit is a chromogenic assay designed for quantitative determination of protein C activity in human citrated plasma
Protein C is a vitamin K dependent plasma protein which plays an important role in the anticoagulant regulatory mechanisms. It circulates as a zymogen and is converted to an active serine protease, activated Protein C (APC), by the action of thrombin in presence of thrombomodulin. APC regulates the coagulation system by proteolytic cleavage and inactivation of activated factors V and VIII. Hereditary and/ or acquired Protein C deficiency has been shown to be a risk factor for development of venous thrombosis. Protein C in plasma is activated by a specific enzyme from Agkistrodon c. contortrix Snake venom. The amount of activated protein C is determined by the rate of hydrolysis of the chromogenic substrate pNAPEP 1566TI. The pNA release measured at 405 nm is proportional to the Protein C level in the range from 0 – 130 % of normal plasma.

Kit contents:

  • 4 vials of Protac® (5 mL)
  • 4 vials of Substrate PNAPEP 1566TI (5 mL)
Features & Benefits
  • Technical validation file
  • Linearity: 0 – 130 (% activity)
  • Detection limit less than 1 %
  • End point or kinetic method
  • The test can be run on fully automated coagulation analyzers by single determinations, giving results within 5 minutes
  • Stable reagents 5 days on analyzers
  • Validated protocols
Protein C is a vitamin K dependent serine protease which, when activated, inhibits coagulation by inactivating the clotting factors V/Va and VIII/VIIIa. Additionally, protein C has been shown to have profibrinolytic activity. Hereditary, heterozygous protein C deficiency has been found to be associated with an increased risk of venous thrombosis and hereditary, homozygous total protein C deficiency has been found in neonates with purpura fulminans. Reduced levels of protein C have been found in association with vitamin K deficiency and during coumarin therapy.