CHROMOGENIC SUBSTRATES FOR tPA
Comparison of the reactivity of selected chromogenic peptide substrates Pefachrome®
|H-(D)Ile-Pro-Arg-pNA, 2HCl||576.3||1.0 mM||25 mg|
|CH₃SO₂-D-HHT-Gly-Arg-pNA, AcOH||658.9||0.26 mM||25 mg|
The Pefachrome® and PNAPEP® line of chromogenic peptide substrates are two lines of high quality substrates, which allow testing of serine proteinases.
Their focus is on enzymes involved in coagulation and fibrinolysis for thrombin, factor Xa, factor XIIa, kallikrein, activated protein C, plasmin and plasminogen-SK.
Chromogenic substrates are synthetic peptides that react with proteolytic enzymes under formation of colour which can be followed spectrophotometrically and the intensity of which is proportional to the proteolytic activity of the enzyme.
Typically, such substrates are composed of 3 to 5 natural or artificial amino acids. They may be N-terminally protected to reduce undesired degradation by aminopeptidases. On their C-termini they are modified so that upon cleavage of the amide bond a chromogenic group is released.
Most commonly used groups are the p-nitroaniline (pNA) which absorbs light of the wavelength of 405 nm.
All chromogenic substrates are stable when stored at 2° C to 8° C.
pNA free content < 0.5 %
Purity grade > 95 %
Expiry date > 1 year
Glass vials 25 mg or 10 µmol
Package Inserts, certificate of analysis supplied.
Material safety Data Sheet (MSDS) supplied.
Prolonged stability following reconstitution (> 3 months)
We can supply milligram to gram.
Discount according to quantities