In blood, VWF propeptide (VWF:PP) have very different half-lives (12 hours versus 2 hours).
Measurement of VWF: PP is an important tool alongside VWF:AG in characterizing the type of VWF deficiency, especially in patients with shortened plasma half-life of VWF.
VWF is synthesized exclusively by endothelial cells and megakaryocytes in the form of pre-pro-VWF, the propetid of which is eliminated by an enzyme, furin, to give a mature VWF.
The mature VWF will be preserved until its release in the Weibel-Palade bodies in the endothelial cells, while the VWF:PP will be secreted in the plasma. VWF:PP is needed for VWF multimerization and storage.
A mutation in VWF:PP results in a lack of high molecular weight VWF.
Not for therapeutic use. For research use only.
- It's a quick test with results in less than 90 minutes
- Excellent stability of all components
- Excellent precision
- Lower detection limit value : 5 mIU / mL
- Measuring range : 2.5 - 120 mIU / mL
- 12 strips with 8 wells coated with an anti-VWF:PP monoclonal antibody
- 1 bottle of anti-VWF:PP monoclonal antibody coupled to an enzyme, 6 mL
- 1 bottle of substrate solution, 12 mL
- 1 bottle of stop solution, 15 mL
- 2 bottles of sample diluent, 25 mL
- 1 bottle of concentrated wash buffer, 100 mL,
- 1 vial of freeze-dried calibration plasma
- 1 vial of freeze-dried control plasma
- 1 plastic frame
- 1 sheet with calibrator and control values
