Thrombin Generation Time (TGT) is an overall hemostasis test that measures the time and amount of thrombin generated in platelet rich or poor plasma.
This test covers 4 important parameters which makes it possible to estimate the potential of an individual to generate thrombin and can individualize hyper or hypo-coagulable phenotypes. This test is also used to monitor conventional treatments for hemophilia and when using bypass therapy which is necessary when inhibitors develop in patients.
The method is based on the cleavage of a fluorogenic substrate in real time by thrombin according to different concentrations of phospholipids and FT.
The concentration of thrombin (nM) in plasma can thus be calculated using a calibration curve. The different phases of TGT formation can thus be visualized. The Technothrombin® TGA kit can be used to monitor the entire hemostatic system. Many additional reagents can be purchased in addition. (Specialized hemostasis)
- 3 vials of TGA substrate (1.5 mL)
- 1 vial of TGA buffer (3 mL)
- 1 vial of TGA thrombin calibrator (0.5 mL)
- 1 vial of low TGA RC reagent: low concentration of phospholipids
+ Recombinant human tissue factor (rhFT) 0.5 mL
- 1 bottle of TGA RC high reagent : high concentration of phospholipids + rhFT 0.5 mL
- 1 vial of TGA RD reagent: phospholipids 1.5 mL
- 2 vials of TGA control (1 CH and 1 CL) : human plasma with increased thrombin generation,
CL : human plasma with decreased thrombin generation 2x1 mL