Belonging to the serine protease family, u-PA activates plasminogen to convert it into plasmin, an enzyme allowing the degradation of fibrin.
It intervenes in the phases of dissolution of the clot during fibrinolysis.
It has also been shown to increase the amount of u-PA in some tumors.
The measurement is based on the u-PA binding to the bottom of the wells thanks to the anti-u-Pa monoclonal antibody, the u-PA will be revealed by a biotinylated anti-u-PA polyclonal antibody which will be detected with streptavidin-HRP and hydrolysis of TMB by HRP will give a stain whose absorbance will be read at 450 nm.
Both single and double urokinase chains are detected. (Specialized hemostasis)
- Stability 6 months after opening.
- Reaction time 200 minutes.
- A calibrator calibrated against NIBSC 87/594 included.
- Sensitivity between 0.6 to 10 ng / mL.
- 12 x 8-well breakable ELISA strips coated with anti-u-PA monoclonal antibody
- 1 vial x biotinylated anti-u-PA polyclonal antibody
- 1 vial x TMB chromogenic substrate (12 mL)
- 1 vial x streptavidin-coupled peroxidase (POX) solution
- 1 vial x dilution concentrate 2.5 x
- 1 vial x dilution buffer (POX)
- 1 bottle x stop solution (15 mL)
- 1 vial x wash buffer (80 mL)
- 1 vial x u-PA calibrator