The measurement is based on the u-PA binding to the bottom of the wells thanks to the anti-u-Pa monoclonal antibody, the u-PA will be revealed by a biotinylated anti-u-PA polyclonal antibody which will be detected with streptavidin-HRP and hydrolysis of TMB by HRP will give a stain whose absorbance will be read at 450 nm.
Both single and double urokinase chains are detected. (Specialized hemostasis)

- Stability 6 months after opening.
- Reaction time 200 minutes.
- A calibrator calibrated against NIBSC 87/594 included.
- Sensitivity between 0.6 to 10 ng / mL.

- 12 x 8-well breakable ELISA strips coated with anti-u-PA monoclonal antibody
- 1 vial x biotinylated anti-u-PA polyclonal antibody
- 1 vial x TMB chromogenic substrate (12 mL)
- 1 vial x streptavidin-coupled peroxidase (POX) solution
- 1 vial x dilution concentrate 2.5 x
- 1 vial x dilution buffer (POX)
- 1 bottle x stop solution (15 mL)
- 1 vial x wash buffer (80 mL)
- 1 vial x u-PA calibrator