Tissue plasminogen activator (t-PA) is a protein involved in breaking down the blood clot. It is a serine protease found in the endothelial cells that line the blood vessels.
Like any enzyme, it converts plasminogen into plasmin, the main blood clot lysis enzyme. Due to its lysis activity, t-PA is used in clinical medicine to treat cerebral embolism and thrombosis.
Its use is contraindicated in cases of cerebral hemorrhage or head trauma.
The bound t-PA converts glu-plasminogen into plasmin which causes, with the substrate, a release of a colored product, the concentration of which is proportional to the quantity of active t-PA.
After washing, the t-PA remains bound to the wells and incubation with the anti-t-PA monoclonal antibody coupled to POX will recognize the active and inactive forms of t-PA.
POX will give the substrate a colored compound whose concentration is proportional to the total amount of t-PA.
T-PA activity : 0.05-10 IU / mL
Antigenic : 0.1 to 20 ng / mL
- 12 strips of 8 breakable wells, coated with anti-t-PA monoclonal antibody
- 2 adhesives for ELISA plate
- 1 vial x anti-t-PA antibody coupled to peroxidase (POX), 0.3mL
- 1 vial x incubation buffer (90 mL)
- 1 vial x wash buffer (80 mL)
- 1 vial x TMB chromogenic substrate (12 mL)
- 1 bottle x stop solution (15 mL)
- 1 vial x dilution buffer (20 mL)
- 1 vial x a mixture for the detection of plasminogen activator coupled to pNa
- 1 vial x recombinant t-PA calibrator