FIX is a vitamin K dependent glycoprotein synthesized by the liver.
The FIX can be activated in FIX in FIXa by the FXIa or by FVIIa in the presence of phospholipids and calcium.
A person who is deficient in FIX has hemophilia B.
- Linearity : 0.005-2 IU / mL
- Two possible curves : linearity between 3 and 25 mIU/mL and between 50 and 500 mIU/mL
- Excellent sensitivity around 0.001 IU / mL with a signal > 30 mA at 405 nm
- No FIX-deficient plasma is used
- CE validated adaptation on STAR, CS5100, BCS XP and ACL Top
The ROX FACTOR IX is a chromogenic enzymatic assay kit for the determination of FIX in human FIX concentrates. FIX insufficiency as well as FIX replacement therapy can be monitored. (Specialized hemostasis)
This method is based on the activation of human FIX by human FXIa.
The FIXa thus formed activates FX to FXa in the presence of FVIII, phospholipids and calcium ions.
The amount of FXa generated is determined by hydrolysis of a chromogenic substrate for FXa.
The quantity of para-nitroaniline released during this hydrolysis and measured at 405 nm is proportional to the concentration of FIXa in the reaction medium.
- 2 vials x reagent A (human FVIII and FX, and bovine FV, lyophilized fibrin polymerization inhibitor)
- 2 vials x reagent B (lyophilized human FXIa and FII, CaCl2 and phospholipids)
- 1 vial x FXa Chromogenic Substrate (6 mL)
- 1 vial x Tris BSA Dilution Buffer (20 mL)
