The conversion of prothrombin to thrombin is a key event in thrombus formation. Thrombin is a serine protease that acts on a wide variety of substrates during the clotting process.
Thrombin generated in vivo can be assessed indirectly by measuring the fragment of prothrombin F1.2, an activating peptide generated during the conversion of prothrombin to thrombin, or thrombin-antithrombin complexes (TAT), formed during inactivation of thrombin by its major inhibitor present in plasma.
However, due to differential accumulation in the circulation, these parameters do not reflect the current state of functional active thrombin in vivo.
In combination with the thrombin blood collection tubes (product ref. 26-ADG844T25 and 26-ADG844T50) which ensure ex vivo stabilization of thrombin activity, the OLIGOBIND® Thrombin activity assay kit allows direct quantification of the level of thrombin.
- Functional active thrombin in blood plasma
- End point or kinetic measurement Low limit of quantification 0.35 mU / mL thrombin
- Specific for human thrombin
- Platelets may interfere with the test
- 12 breakable ELISA strips of 8 wells coated with Aptamers
- 1 bottle x 50 mL washing buffer concentrate
- 2 sets x 6 vials of 0.5 mL calibrators numbered 1 to 6
- 1 bottle x 140 µL fluorogenic substrate
- 1 bottle x 15 mL substrate buffer