Dabigatran is a potent, direct, competitive and reversible thrombin inhibitor. It inhibits free and fibrin-bound thrombin and thrombin-induced platelet aggregation.
DTI : Direct Thrombin Inhibitors
UFH : Unfractionated heparin
LMWH : Low molecular weight heparin
The Technoclot® DTI kit is used for the determination of the anticoagulant activity of the direct thrombin inhibitor (DTI) dabigatran in human citrated plasma.
Dabigatran is an active compound in the oral prodrug, dabigatran etexilate, which has been approved for many indications under the brand name Pradaxa®.
To measure dabigatran in plasma, the patient's plasma is diluted in normal human plasma.
A clot is generated by the addition of thrombin. The clotting time is directly related to the concentration of dabigatran in the sample plasma.
There is no interference from UFH or LMWH up to 1.2 IU / mL.
A correlation coefficient was obtained by comparing the Technoclot DTI kit (Technoclone) with the Hemoclot TI kit (Hyphen) for dabigatran : n = 30 R² = 0.9841.
- 2 vials x 2 mL lyophilized normal plasma
- 2 vials x 2 mL of lyophilized bovine thrombin
