Type I / type III fibrillar collagen. These are the collagens found in the extracellar matrix of our blood vessels.
Von Willebrand factor binds to type I and III collagen fibers through the A3 domain.
Collagen is also a powerful activator of blood platelets by its attachment to its GPVI receptor.
Solcoll can also be used to trigger platelet aggregation in whole blood impedance tests. It is in the form of a relatively stable, slightly cloudy and viscous liquid suspension of 200 μg / ml in 0.02 M of tris / HEPES glucose buffer at pH 7.2.
The 100 µg / ml stock solution can be diluted in water, saline, or neutral buffer of lower ionic strength to any desired collagen concentration.
A range between 1 and 10 µg / ml is usually prepared for light transmission aggregometry (LTA).
Platelet aggregation is usually performed with a dilution of 0.45 ml of platelet rich plasma and 0.05 ml of collagen although proportionately smaller volumes can be used.
- 1 glass vial x 10 mL of liquid collagen
